|
 |
 Home > Available Issues |
|
|
 Original Articles |
| A study on the pathogenicity of Bordetella pertussis in mice (An observation on the target organ for Bordetella pertussis) |
| Younghat Ryu |
| Department of Microbiology, National Institute of Health, Korea |
Vol.2 Num.1 (p41~50)
|
|
|
In testing the potency of pertussis vaccine in mice the intracerebral inoculation method advocated by Kendrick and Eldering has long been used in many countries. The results obtained from their method were found to be correlating closely with the results of the field trial on the effect of vaccination against whooping cough in the United States and in the United Kingdom. But the mice being used for testing protective immunity may hardly be evaluated reasonably, since mice are not the natural host for Bordetella pertussis.
Therefore, probable discrepancies between the sensitivity of the organ and the host itself to Bord. pertussis may be a problem of study for the titration of potency of the vaccine. The organ susceptibility to Bord. pertussis has been reported particularly in the fatal case of human which showed diffuse petechial haemorrhages in the brain tissues and inflammation of interstitial tissues of the lung. If mice were taken as the experimental animal for the measurement of potency of the vaccine, it would be desirable to have certain similarities in the tissue responeses comparable with human organs, although the two species are situated far from each other.
The first attempt was to compare the target organs between those of mice and human beings using various methods of approach for the detection of pathological changes and distribution of Bord. pertussis.
It was found that massive haemorrhagic changes and numerous number of Bord. pertussis appeared first in the brain tissue following the lung tissue to which the organisms were introduced through the route of a caral passage of the intranasal inoculation. Next to the brain, the heart muscle, the kidrey, the liver and the spleen tissues were involved.
The observations were summarized briefly as follows:
1.It was confirmed that the Bordetella pertussis, strain 18~323, maintained in the national institute of Health of Korea was fully virulent possessing the capsule, agglutinogen, particularly K antigen, haemagglutinin, haemolysin, histamine-sensitizing factor and dermonecrotic toxin corresdonding to the Phase I.
2. It was found that the brain was the first target organ for Bordetella pertussis in mice since the haemorrhagic lesions and numerous number of organisms appeared first in the brain tissue even after intranasal inoculation as well as after intracerebral, intraperitorneal and intravenous inoculation of Bord. pertussis prior to any other organ. The next target organ appeared to be the lung suggesting the similarity in the response with the human organs to the aerophilic properties of Bord. pertussis.
3. In connexion with the fatality of mice the pathological changes in the brain might be regarded as anticipating the cause of death, although the pathologicl charges in the lung were more remarkable than the former.
4. It was confirmed that the potency test of pertussis vaccine by the intracerebral inoculation method of Kendrick and Eldering could be evaluated properly since the brain was found to the most susceptible target organ for Bord. pertussis in mice.
|
|
|
Keywords : |
|
|
|
|
|
|
|
|
