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 Original Articles |
| A Report on the Identification of Salmonella Cultures Collected in Korea(1971) |
| Young-Ja Kim, Younghat Ryu |
| Department of Microbiology, National Institute of Health , Seoul, Korea |
Vol.4 Num.1 (p65~70)
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The authors indentified 176 Salmonella cultures either isolated from the apparent cases in various epidemic areas or collected from the Seoul infectious diseases hospital, the provincial hygiene laboratories and the county health centers in order to be confirmed bacteriologically during the period from January to December in 1971 since the preparation for establishing Korean National Salmonella Center was on the pavement to provide the diagnostic service over the country.
The screening procedures for the morphological and biochemical tests, and the serological determinations for the somatic and flagellar antigens as well as Vi antigen were performed according to therecommendations made by U.S. Center for Disease Control. The results of the laboratory tests were summarized as follows:
1. Of 176 Salmonella cultures, 163 Salmonella typhi with Vi antigen and 13 other Salmonella cultures were confirmed.
2. With 163 S. typhi cultures, all typically showed methl red positive, motility positive, acid from glucose positive, mannitol positive. 9.2% showed sorbitol positive, 0.6% showed arabinose positive, 61.4% showed arginine dihydrolase positive and 77.3% showed D-tartrate positive as summarized in Table 1.
3. With 13 Salmonella cultures other than S. typhi, 92.3% showed Simmons' citrate positive, and all produced acid and gas from glucose. All showed sorbitol positive, 84.6% showed dulcitol positive, 23.1% showed inositol positive, 92.3% showed arabinose positive, 69.3% showed rhamnose positive as summarized in Table 1, too.
4. Of 13 Salmonella cultures other than S. typhi, one S. paratyphi A, one S. typhimurium, one S. jericho, one S. colorado, one S. bareilly, three S. berta and five S. enteritidis cultures were confirmed as shown in Table 2 and S. jericho was considered not to have ever been reported in the country before.
5. The sensitivity tests, by means of Ericsson's disc method, to eight kinds of antibiotics were carried out, i.e. chloramphenicol, neomycin erythromycin, colistin, kanamycin, tetracycline, streptomycin, and ampicillin, which were widely in common use in Korea and the result were compared with that of Salmonella cultures isolated during the period from 1967 to 1970 as shown in Table 3. There were three cultures resistant to chloramphenicol and 27 cultures (17%) resistant to ampicillin found among 163 cultures of S. typhi. With 13 cultures of Salmonella other than S. typhi, five cultures were found to be resistant to ampicillin, but none to chloramphenicol.
6. In regards to the multiple resistance to the antibiotics tested, with 163 cultures of S. typhi, all cultures tested showed multiple resistant patterns to more than three kinds of antibiotics in various combinations as shown in Table 4. 152 cultures(ca 93%) showed multiple resistant patterns to more than four kinds of antibiotics in various combination. 128 cultures (79%) showed that to more than five kinds of antibiotics and 33 cultures (18%) showed that to more than six kinds of antibiotics in various combinations including chloramphenicol. And the results were also compared with the results obtained from the previous study during the period from 1967 to 1970 as shown in Table 4.
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