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 Original Articles |
| Large Volume Culture of Peritoneal Dialysate for Microbiologic Diagnosis in Peritonitis during Continuous Ambulatory Peritoneal Dialysis |
| Chang Hyun Choi, Jun Hee Woo, Sang Koo Lee, Dong Cheol Han, Min Sun Park, Hi Bahl Lee |
| Department of Internal Medicine, Soon Chun Hyang University Hospital, Seoul, Korea |
Vol.22 Num.4 (p227~231)
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The rate of identifying microorganisms using large volume culture from peritoneal dialysate was evaluated among 149 episodes of clinical peritonitis during CAPD managed in the Soon Chun Hyang University Hospital, Seoul, Korea.
Three groups of different volumes were compared: group I: 1000 ml divided into 4 equal volumes (n=52), group II: 600 ml divided into 2 equal volumes of 250 ml and 50 ml (n=52), group III, 200 ml divided into 4 equal volumes (n=45).
The results were as follows:
The overall rate of identification was 49.3% (73/149).
In group I, rate of identifying microorganisms was 57.7%, in group II 52.4% and in group III 31.1% respectively (P<0.05, group II versus group III).
Among the isolates, 49.2% were gram positive bacteria, 12.2% gram negative rods and 13.8% fungi. The most frequent isolate was Staphylococcus aureus. (28.7%)
The identifying rate in the dialysate with leucocytes more than 100/mm3 was 52.4% as compared with 41.3% less than 100/mm3.
These results suggest that large volume culture of the dialysate is superior to the small volume (conventional 10 ml) culture.
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