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 Original Articles |
| A Study on Superoxide Anion Production by Peripheral Blood Neutrophil in Patients with Diabetes Mellitus |
| Sang Won Shin, Jae Myung Yu, Se Yong Kang, Heung Jung Woo, Woo Joo Kim, Sung Chull Park |
| Department of Internal Medicine, Korea University, College of Medicine, Seoul, Korea |
Vol.24 Num.4 (p285~291)
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It is generally believed that diabetics have increased susceptibility to infections, and have worse prognosis than nondiabetic population. But there is no evidence that immunologic competence is impaired in patients with diabetes.
This study was designed to investigate the association of the superoxide anion production by peripheral blood neutrophild with the infections and other complications in diabetic patient. Superoxide anion production by peripheral neutrophils in 19 healthy subjects, and 40 diabetic patients (15 with infection and 25 without infection) were measured. The superoxide anion production, measured by spectrophotometric assay was expressed in absorbance at 550 nm. The results were as follows:
1) After stimulation with 10-6 FMLP (N-formyl methionyl leucyl phenylalanine), the superoxide production by neutrophil was higher in normal control group (17.14±3.54 nmole/30 min/1×106 neutrophils) than diabetic patient group (15.60±4.16 nmole/30 min/1×106 neutrophils).
2) Superoxide anion production was lower in the group of diabetic patients with infections (14.58±3.55 nmole/30 min/1×106 neutrophils) than the normal control group (17.14±3.54 nmole/30 min/1×106 neutrophils).
3) Superoxide anion production was lower in the group of diabetic patients with complication (14.13±3.33 nmole/30 min/1×106 neutrophils) than the group of diabetic patients without complication (17.05±4.45 mole/30 min/1×106 neutrophils).
In conclusion, the superoxide anion production defect in diabetic patient have associated with development of infections. And superoxide anion production may appear to have influence on the development of diabetic complication.
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