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 Original Articles |
| Clinical Study of Epstein-Barr Viral Lymphadenitis by Using In-Situ Hybridization Technique |
| Yong Gi Kim, Sung Dong Choi, Jae Kyun Hur, Jin Han Kang, Se Jeong Oh*, Seung Man Park* Young Ha Kim |
| Department of Pediatrics, of General Surgery*, Catholic University Medical College, Seoul, Korea |
Vol.25 Num.4 (p325~331)
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Lymphadenitis in children is usually associated with systemic viral illness. Especially, Epstein-Barr Virus(EBV) may have common causative roles of acute and chronic lymphadenitis in children. However, there were no definitive or practical tools to confirm diagnosis and pathogenesis of EBV lymphadenitis. Fortunately, molecular biological methods, especially in 냐셔 hybridization method, are using now to resolve the problems in recent time.
We started this study to determine the effectiveness of in situ hybridization techinique for thediagnosis and pathogenetic study of EBV lymphadenitis. The case patients were 21 children presented with reactive hyperplasia on biopsied lymph node since Jan., 1990 to Jul., 1992 at Our Lady Mercy Hospital. Postive cellular staining were obtained with NotI PstI oligoprobe related to EBV lytic infection, and EBERs probe related to EBV latent infection in paraffin embeded sections of the cases. 6 positive signals of NotI PstI probe hybridization on B-blast, and 1 positive signal of EBERs probe hybridization on small lymphocyte were seen at lymphoproliferative paracortical area. The children with positive hybridization had history of acute or chronic tenderful lymphadenopathy, usually located posterior cervical region. Also, we confirmed that one EBERs and NotI PstI positive case was converted to malignant lymphoma one year later.
We conclude that this in situ hybridization method with probes related to variable representable status of EBV infection is very useful in diagnosis and pathogenetic study of EBV lymphadenitis.
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