|
 |
 Home > Available Issues |
|
|
 Original Articles |
| In Vitro Effect of Interferon-γ and Granulocyte-Macrophage Colony Stimulating Facter on Peritoneal Macrophages during CAPD |
| Jung Hee Woo, Dong Wha Song, Jee Yun Lee, Dong Cheol Han, Sang Koo Lee, Seung Duk Whang, Hi Bahl Lee |
| Department of Internal Medicine, Soon Chun Hyang University Hospital and Hyonam Kindey Laboratory, Soon Chun Hyang University, Seoul, Korea |
Vol.26 Num.1 (p39~45)
|
|
|
Background: Peritonitis remains the most common cause of CAPD drop out. Reduced activity of peritoneal macrophages (Mφ) may be one of the risk factors for peritonitis during CAPD. Interferon-γ (IFN-γ) and Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) have been shown to control Mφ activity in animal and in vitro experiments. This study was designed to evaluate the effect of IFN-γ and GM-CSF on peritoneal Mφ during CAPD.
Methods: Mφ were separated from overnight peritoneal effluent from eleven CAPD patients using modified Ficoll-Hypaque methods. The 1×106/mL of peritoneal Mφ were incubated with 104 units/mL of IFN-γ or 40 μg/mL of GM-CSF. Peritoneal Mφ HLA-DR expression, IgGFcIII receptor(FcγRIII) expression, and bacterial phagocytosis were measured before and after incubation with IFN-γ or GM-CSF by two color flow cytometry using monoclonal antibodies.
Results: 1. HLA-DR expression did not change after IFN-γ but increased after GM-CSF, although statistically insignificant. 2. Mφ FcγRIII expression did not change after GM-CSF but increased after IFN-γ, although statistically insignificant. 3. Bacterial phagocytosis utilizing FITC labeled S. aureus increased after IFN-γ (P<0.05) and increased also after GM-CSF, although statistically insignificant.
Conclusion: INF-γ and GM-CSF augmented the peritoneal macorphage activity as shown by significant increase in bacterial phagocytosis and by tendency to enhanced expression of HLA-DR, and FcγRIII expression.
|
|
|
Keywords : CAPD, Peritoneal defense mechanism, Peritoneal Mφ, IFN-γ, GM-CSF. |
|
|
|
|
|
|
|
|
