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 Original Articles |
| Bactericidal Effect of Disinfectant Solution Produced by Bacteriokiller System |
| Jong Sung Suk, Hyeon Soo Kim, Eui Chong Kim |
| Department of Clinical Pathology, Seoul National University College of medicine, Seoul, Korea |
Vol.27 Num.6 (p553~557)
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Background : Disinfection is essential for the prevention of hospital infection. Bacteriokiller(BK) system has been recently developed as an apparatus shich produces disinfectant. Handwashing solution from BK system shows sterilizing effect against clinical isolates by the action of active oxygen and hypochlorous acid. We evaluated the disinfective activity of this product against many clinically isolated strains of bacteria and yeast.
Methods : 31 strains of bacteria and a strain of yeast were exposed to 20 ppm BK disinfectant for various periods (0.5, 1, 2, 4, 8 minutes). After exposure of BK disinfectant, 0.01 mL of mixture of bacteria and BK disinfectant was incubated in BHI broth or Sabouraud dextrose agar, at 37℃ for 48 hours and the growth of bacteria was examined.
Results : all strains of bacteria and yeast except for B. subtilis were eradicated within 30 seconds after exposure of BK disinfectant. Spore forming B. subtilis were killed in four minutes exposure.
Conclusion : This study showed that BK disinfectant was effective for the disinfection of commonly isolated bacteria and some yeast from hospital. Spore forming bacillus species need a longer exposure time to be eradicated.
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Keywords : Disinfectant, Bacteriokiller system, Hospital infection |
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