|
 |
 Home > Available Issues |
|
|
 Original Articles |
| The Effect of the Antithrombotic Polyurethanes on the Colonization of Staphylococcus epidermidis |
| Kyong Ran Peck,
Sungmin Kim, Kyu-Back Lee*, Myoung-Don Oh, Byung-Goo Min*, Kang-Won Choe |
| Department of Medicine, Samsung Medical Center, Seoul Korea
Department of Internal Medicine, Department of Medical Engineering*, Seoul National University College of Medicine, Seoul, Korea |
Vol.28 Num.1 (p31~40)
|
|
|
Background : Infection and thromboembolism are major complications of prolonged use of prosthetic devices, such as various catheters, prosthetic joints, and artificial hearts, frequently resulting in severe morbidity, or death. Therefore, the preventicn of infection and thrombosis is an important subject in developing new biomaterials of prosthetic devices.
The first event on insertion of a prosthetic device into the blood is protein adsorption and activation with platelet adhesion, activation and aggregation, leading to the formation of platelet-fibrin thrombi. The thrombi are susceptible to bacterial incorporation and protect the bacterial from the effects of circulation antibodies, phagocytic cells, and antibiotics. Furthermore, adherent bacteria may induce fibrin and platelet adhesion.
Sulfonated polyurethane and albumin-immobilized polyurethane were documented to diminish the adhesion and shape-change of platelet on the polymer in comparison with plain polyurethane, in vitro. The objective of this study was to evaluate the effect of these antithrombotic biomaterials on the bacterial colonization.
Methods : Plain, sulfonated, albumin-immobilized, and paraben-incorporated polyurethane(PU) films were prepared as a disk form. Staphylococcus epidermidis RP12 was suspended at a concentration of 107/mL and four types of polymeric disks were immersed in the bacterial suspension. After incubation for 24 and 48 hours, the bacterial colonization on the each disk was determined by the colony forming units(CFU) counting method. The colonized disks were examined by scanning EM.
Results : The paraben decreased significantly the number of CFU on PU surface to 2.1×104/mL after 48 hours, compared with 1.1×105/mL on plain polymers. The number of CFU on sulfonated or albumin-immobilized PU was 8.2×104/mL, and 1.4×106/mL, respectively. SEM micrographs showed no reduction of colonization on antithrombotic PUs.
Conclusion : Antithrombotic treatments of polymers did not decrease the bacterial colonization on polymers, in vitro, that was compared to the decreasing effect of paraben. However, because the thromib and bacteria interact with each other, the overall effect of the antithrombotic polyurethanes on the bacterial colonization and infection should be studied in vivo.
|
|
|
Keywords : Antithrombotic polyurethane, Biomaterial, Staphylococcus epidermidis, Colonization |
|
|
|
|
|
|
|
|
