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 Original Articles |
| Pulsed-Field Gel Electrophoresis Pattern, Phage Type and Drug Susceptibility of Salmonella typhi Isolates from an Outbreak in Pusan City in 1996 |
| Young Hack Shin/Jung Sik Yoo/Bok Kwon Lee/Ho Hoon Kim/Mi Sun Park/Yeun Ho Kang |
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Vol.29 Num.3 (p201~208)
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Background : During the period from April to June 1996, an outbreak of typhoid fever
occurred in Suyoung-Ku, Pusan city. We performed the molecular epidemiological
analysis and drug susceptibility test for 48 isolates of Salmonella typhi from the
outbreak.
Methods : Chromosomal DNA of S. typhi was digested with the restriction
endonuclease Spe I and the resulting restriction polymorphism was observed on pulsed
field gel electrophoresis(PFGE). Phage typing was performed using Vi-phages, and
antimicrobial susceptibility test was done by Disc diffusion method.
Results : On phage typing, 2 isolates were Ml phage type, 2 El phage type and 44
untypable. The PFGE analysis on 14 to 16 chromosomal DNA restriction fragments
ranged from 50Kb to 1000Kb showed 2 different patterns to restriction fragments,
divided into group A and B. Forty-seven isolates belonged to group A that were
subgrouped into Al(42 strains), A2(1 strain), A3(3 strains), A4(1 strain), and one strain
was grouped into group B. In the F-value of genetic similarity of the epidemic S. typhi
strains, there was low similarity(F value : 0.2-0.258) between group A strains and group
B strains. However, the subgroup strains(Al-A4) showed high similarity(F value
0.8-0.897) each other. The drug susceptibility test showed susceptibility to ampicillin,
carbenicillin, cephalothin, chloramphenicol, tobramycin, gentamycin, kanamycin, nalidixic
acid, neomycin, polymyxin B, streptomycin, tetracycline, trimethoprim-sulfamethoxazole,
ciprofloxacin and norflokacin.
Conclusion : This study showed that phage typing and PFGE were very useful as a
tool to investigate milecular analyes of epidemic S. typhi strains this study. |
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Keywords : Salminella typhi, Phage typing, Pulsed-field gel electrophoresis, antimicrobial, susceptibility |
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