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 Original Articles |
| Species Identification of Enterococcus by Ribotyping |
| Park YJ, Huh DH, Kim BK, Kim SM, Shim SI |
| Department of Clinical Pathology, Clinical Research Institute of Catholic University, Medical College, Seoul, Korea. |
Vol.29 Num.5 (p377~386)
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BACKGROUND: Emerging drug resistance and increasing incidence of enterococci infection necessitates an accurate species identification.
METHOD: We compared the identification of twelve strains of American Type Culture Coliection(ATCC) (E. faecalis, E. avium, E. faecium, E. raffinosus, E. durans, E. casseliflavus, E. hirae, E. flavescens, E. malodoratus, E. gallnarum, E. mundtii, and E. sulfureus) and 73 clinical isolates of enterococci(E. faecalis 8, E. faecium 8, E. avium 13, E. durans 5, and unidentified strains 39) by Vitek Gram-positive Identification card (version R08.1, bio- Merieux Vitek, Hazelwood, Mo., U.S.A.), ribotyping and conventional scheme of Facklam and Sahm.
RESULTS: All ATCC strains could be identified on the basis of characteristic 16S-23S rDNA fingerprint patterns by using Eel I and Hind III. Of 39 strains unidentified by Vitek GPI card, 25 strains were identified as E. faecium, 5 strains were E. gallinarum, 5 strains were E. casseliflavus, 3 strains were E. avium and 1 strain was E. hirae.
CONCLUSION: By ribotyping with Eel I and Hind m digestion, all twelve ATCC strains and 73 clinical strains were identified and it seems to be a reliable method for identification of Enterococcus species.
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