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 Home > Available Issues |
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 Original Articles |
| Mechanism of Toxic Shock Syndrome Toxin-1
as a Superantigen |
| Jun-Hee Woo, M.D., Eun Ok Kim, M.D., Yang Soo Kim, M.D.,
and Jiso Rhu, M.D. |
| Division of Infectious Diseases, Department of Internal Medical, Asan Medical Center
University of Ulsan College of Medicine, Seoul, Korea |
Vol.30 Num.2 (p134~142)
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Background:Staphylococcus aureus TSST-1 is a microbial superantigen which requires interaction between MHC class II molecules and T-cell receptors. To characterize the mechanism of TSST-1 as a superantigen, we investigated the influence of TSST-1 on the binding of T-cell receptor and MHC II.
Methods:For control, A20 cells and lymphocytes from healthy volunteers were measured by the IL-2 bioassay with or withtout TSST-1, which was then purified and confirmed using immunoblot. mRNA for TNF from PBMC was prepared and RT-PCR for mRNA expression was performed.
Results:When stimulated by TSST-1(100pg/ mL), lymphocytes produced 135μg/ml of IL-2 compared to the control A20 cells which produced 5μg/ ml of 1L-2. TSST-1 was confirmed to be the strong inducer for mRNA expression by RT-PCR.
Conclusion:The binding to T-cell receptor was enhanced by TSST-1 as confirmed by the bioassay of IL-2. This data may contribute to the understanding of mechanism of cell-toxin interaction. |
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Keywords : TSST-1, mechanism, superantigen |
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