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 Home > Available Issues |
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 Original Articles |
| Effects of Addition of Sugars on the Stability of Leptospira Vaccine |
| In Wha Seong |
| Department of Microbiology, Medical College, Korea University, Seoul, Korea. |
Vol.30 Num.6 (p551~557)
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Background : Most of the current licensed vile and bacterial vaccines produced in the world are in the state of antigen suspension, and the immunogenicities of the vaccines can be maintained for one to two years at 4 ℃, but without refrigeration vaccines easily lose their immunogenicities.
Methods : In this study, as an attempt to improve the stability and maintenancee of
the immunogenicity of the vaccines at room termperature or at higher temperatures,
sucrose, lactose and glucose were added into the leptospira vaccines respectively, and kept at 37 ℃ for 1, 3, 5, and 7 days. The vaccines were inoculated twice into Sprague Dawley rats after 7 days' interval and the titers of the antibodies against the antigen of Leptospira icterohemorrhagiae in the sera from rats were measured. Results were evaluated by logistic regress analysis (SAS/PC).
Results : Sera from rats inoculated with the normal control vaccine kept at 4 ℃ for 7
days showed antibody titers of 1:20∼80. The vaccine without sugar exposed at 37 ℃ for one day nearly lost its immunogenicity. And the vaccine contained sucrose could keep the immunogenicity at least for 7days at 37 ℃ (p=0.001), though the titers were slightly lower than the titer of normal control. Stabilities of lactose and glucose were lower than sucrose.
Conclusion : Addition of sucrose, lactose and glucose could increase the stability of leptospira vaccine. 7.5% fad 10% sucrose in vaccine could maintain the immunogenicity of leptospira vaccine for 7 days at 37 ℃. This method is expected to be applied to the production of other viral and bacterial vaccines.
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Keywords : Leptospira vaccine, Stability, Sugars |
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