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 Original Articles |
| Mechanism of β-lactam Resistance of Gram-negative Rods Isolated from Surgical Site Infection |
| 1Haeng Seop Shin/2Yong Shin Kim/1Dong Taek Cho |
| Department of Microbiology, College of Medicine, Kyungpook National University, Taegu, Korea. Department of Surgery, Kangbuk Samsung Hospital, Seoul, Korea. |
Vol.31 Num.5 (p390~401)
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Background : There have been few studies about the kinds of species causing surgical site infections and their resistance pattern in Korea. An increase of extended-spectrum β-lactamase (ESBL) producing strains is a worldwide problem. However, there is not enough data on the prevalence of ESBL-producing strains in Korea and the true extent of this problem seems to be under-recognized.
Methods : Minimal inhibitory concentrations of gram-negative bacilli isolated from surgical site infections were tested using the standard agar dilution method according to the National Committee for Clinical Laboratory Standards. To identify and characterize beta-lactamases, we performed conjugation test, isoelectric focusing, Southern hybridization, and polymerase chain reaction.
Results : A total of 54 strains of gram-negative enteric bacilli were identified : two strains of Acinetobacter spp., one of Citrobacter freundii, nine of Enterobacter cloacae, one of Enterobacter sakazakii, one of Escherichia coli, two of Klebsiella pneumoniae, one of Morganella morganii. one of Proteus vulgaris,23 of Pseudomonas aeruginosa, four of Xanthomonas maltophila, and nine of Serratia marcescens. Three straons produced ESBL.
Conclusion : Various species of gram-negative organisms isolated from surgical site infections showed complex antibiograms to various β-lactams, even to the new generation of antibiotics. A large proportion of these strains showed conjugally transferable, plasmid-mediated, β-lactam resistance. Some strains were ESBL-producing. This evidence suggests that there has been a molecular evolution of β-lactamase genes to a great extent in Korea, possibly due to indiscriminate use of antibiotics.
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Keywords : Drug resistance, Beta-lactamase, Extended-spectrum Beta-lactamase, Isoelectric focusing, Gram-negative bacteria |
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