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 Original Articles |
| Usefulness of PCR Method for Identification of Enterococci Species |
| Yeon-Hwa Choi, Yeong-Sun Lee, Hong-Bin Kim, M.D.
Chi-Kyung Kim* and Bong-Su Kim |
| Laboratory of Nosocomial Pathogens, Department of Microbiology, National Institute of Health, Seoul and
Department of Microbiology*, College of Natural Science, Chungbuk National University, Cheongju, Korea |
Vol.33 Num.2 (p123~127)
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Background:Enterococci are important cause of nosocomial infections. Recently, vancomycin-resistant enterococci (VRE) has been increasingly reported as significant nosocomial pathogens. Therefore, accurate identification of enterococcal species is a prerequisite step for the appropriate antibiotic treatment and epidemiologic surveillance. We wanted to know the usefulness of PCR method compared with Vitek automatic identification system.
Methods:Totally 105 isolates were identified on the species level by Vitek (GPI card and software version R06.1), methyl-α-D-glucopyranoside test, and PCR methods.
Results:Among 105 enterococcal isolates, 59 were identified as E. faecium, 11 E. faecalis, 6 E. gallinarum by Vitek. But 29 isolates (28%) were unidentified. Subsequently all of these isolates were analyzed by PCR, the results of which were as follows:17 E. faecium, 5 E. casseliflavus, 7 E. gallinarum. Two isolates identified as E. gallinarum by Vitek were reidentified as E. casseliflavus by PCR and other methods for phenotypic characterization.
Conclusoin:PCR method was more accurate and sensitive than Vitek for the identification of enterococci species. |
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Keywords : Enterococci, Identification, PCR, Vit다 |
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