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 Original Articles |
| Genotypes of Metallo-β-Lactamases Produced by Imipenem-Resistant Acinetobacter spp. Isolates from Clinical Specimens |
| Kim, Ji Hye/Jeong, Seok Hoon/Kim, Bit Na/Jeong, Tae Jeon/Kim, Taek Sang/Kim, Jong Chul/Jeon, Byung Chan |
| Department of Internal Medicine, Kosin University Collage of Medicine, Busan, Korea. Department of Laboratory Medicine, Kosin University Collage of Medicine, Busan, Korea. Department of Urology, Kosin University Collage of Medicine, Busan, Korea. Department of Neurosurgery, Kosin University Collage of Medicine, Busan, Korea. Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea. |
Vol.34 Num.6 (p360~366)
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Background : Carbapenem-resistant Acinetobacter´ spp. are being isolated with increasing frequency from clinical sources. This study was designed to determine the resistance mechanism to carbapenems of Acinetobacter spp. isolates from patients of a tertiary care hospital in Busan, Korea.
Methods : Nonduplicated clinical isolates of Acinetobacter spp. resistant to carbapenems were collected during the period of 2000 2001 in Kosin Medical Center, Busan, Korea. Antimicrobial susceptibilities were tested by disk diffusion method. Carbapenem-resistant Acinetobacter spp. isolates were examined for metallo-,3 -lactamase-production by modified Hodge and EDTA-disk synergy tests. For the detection of bla[mp-, and blav,M-2 genes, polymerase chain reactions (PCR) were performed, and the DNA sequences of amplified products were determined by using dideoxychain termination method.
Results : Among 21 clinical isolates of Acinetobac-
ter spp. intermediate or resistant to carbapenems, 17 isolates showed positive reactions in modified Hodge and EDTA-disk synergy tests. Nine isolates showed positive reaction in PCR for the detection of blaimp-, genes, and 8 isolates showed positive reaction in PCR for the detection of blavtM-2 genes. Sequencing of amplified products showed that they were blaimp-, or blav]M-2 genes.
Conclusion : Acinetobacter spp. isolates with carbapenem-resistance are not uncommon in Kosin Medical Center, and most of the carbapenem-resistant isolates acquired resistance by production of IMP-1 or VIM-2 metallo- Q -lactamases. The spread of metallo-Q -lactamase genes could compromise the future use-fulness of carbapenem for the treatment of gram-negative bacilli infections. |
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Keywords : Acinetobacter, Metallo-β-lactamase, IMP-1, VIM-2 |
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