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 Original Articles |
| Evaluation of CHROMagar Staph aureus, a NewChromogenic Medium, for the Detection of Nosocomial Methicillin-Resistant Staphylococcus aureus |
| Lee Hyuck |
| Division of Infectious Diseases, Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea. |
Vol.34 Num.6 (p367~372)
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Objective : Trichomonas vaginalis is the common cause of sexually transmitted diseases. The present study was performed to find the possibility of other transmission mode of T. vaginalis than sexual . trans-mission.
Methods : Survivals of trophozoites suspended in various environmental conditions were measured by haemocytometer after trypan blue staining. Also, drying time of vaginal secretion exposed at different temperatures such as 41C, 261C, 301C were observed.
Results : The survival rates of T. vaginalis de-creased as the temperatures of tap water increased. The survival rates of trophozoites were less than 10% at 30 min-exposure at 41C or 15 min-exposure at 26 *C water. Hot water above 45 *C killed trichomonads in 5 minutes or so. T. vaginalis soaked in water from swimming pool and in cleaning solution deceased in about 5 minutes. When trophozoites were put into´ urines of six healthy person, the survival rates of T. vaginalis showed less than 10% after 24 hr exposure except KT4. The survival rates of trichomonads werechanged according to individual urine on examined day, and isolate of T. vaginalis. The vaginal secretion was put on slide glass and leave alone until complete drying in 41C refrigerator, 26 C and 301C incubator. For drying of vaginal secretion, it took 70 minutes, 44 minutes and 26 minutes in 41C refrigerator, 261C and 301C incubators, respectively. The survival of trichomonads showed no change until complete dryness of vaginal secretion. T. vaginalis immersed in tap water for 5 minutes, was divided into two or many fragments. Some trichomonads were partially or completely destructed.
Conclusion : From above results, it is supposed that transmission of T. vaginalis by contaminated fomites such as toilet stool, toilet seats is possible al-though this type of transmission may not occur frequently. |
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Keywords : CHROMagar Staph aureus, Methicillin-restistant Staphylococcus aureus |
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