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 Original Articles |
| Use of RNA/RNA Duplex, Base Pair-mismatch Assay and
3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT)
Assay for Rapid Detection of Rifampin-resistant Mycobacterium tuberculosis |
| Sung Kwan Hong, M.D., Kyung Hee Chang, M.D., Yoon Soo Park, M.D., Jeong Ho Cho, M.D.,
Hyo Yeol Kim, M.D., Young Goo Song, M.D. and June Myung Kim, M.D. |
| Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea |
Vol.35 Num.1 (p12~17)
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Background:Multidrug-resistant tuberculosis is an increasing concern for public health in many parts of the world. We have evaluated the specificity and sensitivity of the mismatch assay and 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in detecting rifampin- resistant Mycobacterium tuberculosis.
Methods:Eleven rifampin-susceptible and 15 rifampin-resistant M. tuberculosis strains were isolated from clinical specimens obtained from patients in Yonsei University College of Medicine, Severance Hospital. RNA/RNA duplex, base pair-mismatch assay (Mismatch Detect II kit, Ambion) and MTT assay were performed.
Results:The specificity and sensitivity of detection of rifampin resistance were 91% and 87% in mismatch assay and 73% and 67% in MTT assay, respectively.
Conclusion:These results suggest the usefulness of mismatch assay in detecting rifampin-resistant Mycobacterium tuberculosis. |
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Keywords : Rifampin-resistant Mycobacterium tuberculosis, Mismatch assay, MTT assay |
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