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Immunoreactivity of Iron-Repressible Cell Wall Proteins and Exoproteins of Staphylococcus aureus
Yong Lim, M.D.1, Jong Hoon Park, M.D.1, Seung IL Lee, M.D.2 Soo Young Cho, M.D.3 and Sung-Heui Shin, M.D.1,4
Department of Microbiology1, Internal Medicine2 and Emergency Medicine3, and Research Center for Resistant Cells4, Chosun University Medical School, Kwangju, Korea
Vol.36 Num.4 (p241~244)
In the present study, we tried to investigate immunoreactivity of iron-repressible cell wall proteins and exoproteins of Staphylococcus aureus when the convalescent serum obtained from the patient with septicemia was applied. On SDS-PAGE and western blot analysis, several iron-repressible cell wall proteins expressed in iron-deficient BHI showed strong immunoreactivity, whereas no or relatively weak immunoreactivity was shown in iron-sufficient BHI. Several exoproteins were expressed only in iron-deficient BHI and these exoproteins showed strong immunoreactivity. These results indicate that several iron-repressible cell wall proteins and exoproteins are expressed and are immunogenic in vivo. Since in vivo state is an relatively iron-restricted condition, we recommend the use of iron-deficient media for studies concerning pathogenicity of staphylococcal in human.
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