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 Original Articles |
| VIM-2 Type Metallo-β-lactamase Producing Achromobacter xylosoxidans subsp.xylosoxidans Isolated from Urine Specimens |
| Kyeong Seob Shin, M.D.1, Jungnam Lee2 and Byeong Chan Kim, MD.3 |
| 1Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju,2Department of Microbiology, Dankook University, Cheonan, Korea3Department of Pediatrics, College of Medicine, Seoul National University, Seoul, Korea |
Vol.37 Num.1 (p22~28)
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Background:The dissemination of metallo-β-lactamase (MBL) producing gram-negative bacilli is of great concern because MBL can hydrolyze carbapenem. We report herein the infection by VIM-2 type MBL producing Achromobacter xylosoxidans subsp. xylosoxidans.
Materials and Methods:For seven A. xylosoxidans subsp. xylosoxidans with reduced imipenem susceptibility, the detection for MBL was performed using EDTA double disk synergy test (EDTA- DDS) and the PCR for IMP-1, VIM-1 and VIM-2 genes. The minimal inhibitory concentration (MIC) of MBL producers were determined by microbroth dilution methods. The DNA fingerprinting analysis was performed by random amplified polymorphic DNA.
Results:All seven isolates were MBL producers when tested with EDTA-DDS test and these isolates were VIM-2 type confirmed by the PCR and DNA sequencing analysis. The MIC against imipenem ranged from 16 to 32 μg/mL in these isolates. The DNA fingerprints of these isolates were identical.
Conclusion:A. xylosoxidans subsp. xylosoxidans manifest resistance against imipenem by acquisition of VIM-2 type MBL. To our knowledge, this is the first report on the VIM-2 type MBL producing A. xylosoxidans subsp. xylosoxidans. |
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Keywords : Achromobacter xylosoxidans subsp. xylosoxidans, VIM-2, Metallo-β-lactamase, Carbapenem, RAPD |
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