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Original Articles
Molecular Epidemiological Typing of Methicillin-Resistant Staphylococcus aureusUsing Random Amplified Polymorphic DNA Analysis
Hee-Jae Jun, M.D.1, Jeong-Man Kim, M.D.2 and Jong-Soo Woo, M.D.3
1Department of Thoracic & Cardiovascular Surgery, Pusan Paik Hospital, College of Medicine, Inje University,2Department of Laboratory Medicine, 3Department of Thoracic and Cardiovascular Surgery,College of Medicine, Dong-A University, Busan, Korea
Vol.37 Num.1 (p39~46)
Background:The random amplified polymorphic DNA (RAPD) analysis was investigated to see if this method could be a useful tool for monitoring of epidemic outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) among patients and healthcare workers (HCW) in the intensive care units (ICU).
Methods:Thirty-eight MRSA strains were isolated from patients and HCW in Dong-A University Hospital ICU from October, 1998 to December, 1998 (10 patients and 8 HCW) and May, 2001 to July, 2001 (15 patients and 5 HCW). All strains were typed according to antimicrobial susceptibility and RAPD analysis patterns. mecA genes were detected using polymerase chain reaction (PCR).
Results:Twenty one of 25 (84%) and 12 of 13 (92%) MRSA, isolated from patients and HCW, respectively, were mecA positive. mecA positive MRSA were classified into 18 different types by RAPD analysis.
Conclusion:DNA fingerprinting using RAPD analysis is a simple, effective, and rapid method for discriminating MRSA strains, and may be applicable in detecting outbreaks of S. aureus infections in the ICU.
Keywords : Methicillin-resistant Staphylococcus aureus, Random amplified polymorphic DNA analysis