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Original Articles
The Regulation of Procalcitonin Production inHuman Peripheral Blood Mononuclear Cells
Hee Jung Choi, M.D.1,2, Min-Sun Cho, M.D.2,3, and You Jin Lee, B.A.2
1Department of Internal Medicine, 2Ewha Medical Research Center, Ewha Womans University3Department of Pathology, Ewha Womans University College of Medicine, Seoul, Korea
Vol.38 Num.1 (p11~16)
Background:Procalcitonin (PCT) is a new marker of severe systemic bacterial infection. PCT consists of fragments katacalcin and calcitonin, which are precursors of calcitonin in thyroid. The source and role of PCT in pathogenesis of sepsis remains clarified. This study was focused on which subsets of peripheral blood mononuclear cells (PBMC) can induce PCT when they are stimulated with endotoxin or phorbol myristate acetate (PMA), and how the PCT production is controlled.
Materials and Methods:PBMC were isolated and incubated overnight in each media containing 1 ug/mL lipopolysaccharide (LPS), or 5 ng/mL PMA. Intracellular PCT was detected using fluorescein isothiocyanate (FITC) labeled anti-katacalcin antibody (Ab). Monocytes and lymphocytes were identified by phycoerythrin-conjugated CD14 Ab and CyChrome-conjugated CD3 Ab, respectively. Ten micrograms of soluble TNF receptor (sTNFR) 7
Keywords : PBMC, Procalcitonin, LPS, TNF-?