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Rapid Detection of Extended Spectrum ?-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method
Junyoung Kim1, Semi Jeon1, Hogeun Rhie2, Bokkwon Lee1, Misun Park1, Hoanjong Lee, M.D.3, Jina Lee, M.D.4 and Seonghan Kim1
Division of Enteric Bacterial Infections1, Center for Infectious Disease, National Institute of Health, Institute of Global Environment and Department of Biology 2, Kyung Hee University, Department of Pediatrics3, Seoul National University Children's Hospital, Department of Pediatrics4, Seoul National University Boramae Hospital, Seoul, Korea
Vol.41 Num.3 (p181~184)
A multiplex PCR method has been developed to classify extended spectrum ?-lactamase (ESBL) and plasmid- mediated AmpC ?-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHA type ?-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method. In testing for 73 ESBL- producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of ?-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.
Keywords : Extended Spectrum-? Lactamase (ESBL), Rapid classification, Multiplex PCR