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 Original Articles |
| Characteristics of CTX-M Type Extended Spectrum ß-lactamase Producing Non-typhoidal Salmonella Isolates |
| Soon-Ho Park, Yiel-Hea Seo, Jeong-Yeal Ahn, Pil-Hwan Park, Kyung-Hee Kim, Young-Hee Song and Jung-Eun Kim |
| Department of Laboratory Medicine, Gachon University Gil Hospital, Incheon, Korea |
Vol.42 Num.1 (p35~38)
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Background: Extended-spectrum ß-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital.
Materials and Methods: Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing
Results: Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime.
Conclusions: ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M. |
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Keywords : Legionella pneumophila, Diagnosis, Biomarker, cDNA microarray |
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