|
 |
 Home > Available Issues |
|
|
 Note |
| Further Evaluation of Multiplex PCR for Rapid Detection of Salmonella Typhi |
| Deog-Yong Lee, Jung-Eun Min, Esther Lee, Sung Hun Kim, Hee-Bok Oh, and Mi-Sun Park |
| Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korea Centers for Disease Control and Prevention, Seoul, Korea |
Vol.42 Num.4 (p237~240)
|
|
|
|
Typhoid fever is a class I legally designated communicable disease in Korea; and if remains as an important public health problem in many developing countries. It takes at least 3-5 days to detect and identify Salmonella Typhi (S. Typhi) by classical diagnostic method. For this reason, multiplex PCR (mPCR) was evaluated in detecting and identifying S . Typhi. In this study, forty-three bacterial strains, which consisted of 42 Salmonella enterica serovars and one Citrobacter freundii . were used to evaluate the promptness of mPCR in detecting and identifying S . Typhi. mPCR was performed with four genes which were known for representing Salmonella spp and/or S . Typhi: invA, fliC-d, viaB and prt. invA and prt gene was amplified in all strains and viaB gene was in only S. Typhi. fliC-d gene was amplified in three serovars: S . Typhi, S. Schwarzengrund and S. Livingstone. After specificity test, mPCR was modified as triplex PCR with three genes (invA, fliC-d, and viaB) and the sensitivity test was performed against S. Typhi-inoculated stool samples. mPCR was able to detect S. Typhi cell suspension of 1×105 cfu/mL. We found that modified multiplex PCR was useful to detect S. Typhi from stool samples within 24h whereas it takes 3-5days to detect by classic diagnosis method. |
|
|
Keywords : Salmonella Typhi, Multiplex PCR, invA, fliC-d, viaB |
|
|
|
|
|
|
|
|
