|
 |
 Home > Available Issues |
|
|
 Original Articles |
| Effects of Peroxisome Proliferator-Activated Receptor-γ on the Production of Tumor Necrosis Factor-α in Stimulated Human Monocoytes |
| Eun-Young Kwon1, Chulmin Park1, Jae-Cheol Kwon2, Si-Hyun Kim2, Sun Hee Park2, Su-Mi Choi2, Dong-Gun Lee2, Jin-Hong Yoo2, and Jung-Hyun Choi2 |
| 1Catholic Research Institutes of Medical Science, 2Department of Internal Medicine, Division of Infectious Diseases, College of Medicine, Catholic University of Korea, Seoul, Korea |
Vol.42 Num.5 (p291~295)
|
|
|
Background: We evaluated the effects of peroxisome proliferator-activated receptor-γ (PPAR-γ) on the production of tumor necrosis factor-α (TNF-α) and expression of nuclear factor-κB (NF-κB) in stimulated THP-1 cells, a human monocyte cell line.
Materials and Methods: We evaluated the cytotoxic effect of 15-Deoxy-Δ12,14- prostaglandin J2 (15d-PGJ2), one of natural PPAR-γ ligands, using commercial cell proliferation assay. Cells were pretreated with 15d-PGJ2 and then stimulated with lipopolysaccharide (LPS) or lipoteichoic acid (LTA). The amount of TNF-α was measured by using commercial ELISA method. NF-κB activation was evaluated by Western blot analysis.
Results: 15d-PGJ2 showed dose-dependent cytotoxic effect on the tested cells after 4 hr of treatment. Stimulation of cells by LPS or LTA induced TNF-α production. TNF-α production was markedly decreased in the cells pretreated with 15d-PGJ2compared to cells treated only with LPS or LTA in a dose-dependent manner. Pretreatment of 15d-PGJ2 reduced LPS or LTA induced NF-κB expression in the nuclear extracts of THP-1 cells.
Conclusion: 15d-PGJ2 pretreatment decreased TNF-α production from the THP-1 cells stimulated by LPS or LTA, and this assumed to be associated with inhibition of NF-κB activation. |
|
|
Keywords : Peroxisome proliferator-activated receptor-γ (PPAR-γ), Tumor necrosis factor (TNF)-α, Nuclear factor-κB (NF-κB) |
|
|
|
|
|
|
|
|
