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 Original Articles |
| Clinical Efficacy Evaluation of Multi-parameter Realtime Polymerase Chain Reaction for the Central Venous Catheter-related Blood Stream Infection |
| Bongyoung Kim1*, Sewoo Park1*, Taehyung Kim1, Jieun Kim1, Donghwi Rim1, Taeyeal Choi2, Hyunjoo Pai1†, and Jungoak Kang2† |
| Departments of 1Internal Medicine and 2Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea |
Vol.43 Num.3 (p240~244)
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Background: The study evaluated the clinical efficacy of a multi-parameter realtime polymerase chain reaction (PCR) test for patients with central venous catheterrelated bloodstream infection (CRBSI).
Materials and Methods: Thirty five patients suspected to have CRBSI were enrolled. The SeptiFast™ (SF) multi-parameter real-time PCR test (Roche Diagnostics, Germany) and blood culture were performed and results were compared.
Results: The turn-around time for the SF test and blood culture was 32.6±28.9 hours and 115.8±23.5 hours, respectively. Among the 70 blood samples, the positive rates of SF test and blood culture were 34.3% and 27.1%, respectively, and the agreement rate was 62.9%. Gram-positive bacteria were detected in 10 patients with blood culture and 11 patients with SF test. Gram-negative bacteria were detected in one patient with a blood culture and in seven patients with SF test. Candida was not detected in blood culture but was detected in two patients by the SF test.
Conclusions: SF test was faster and more sensitive for the detection of blood pathogens than blood culture. It provides a more sensitive detection of gramnegative and Candida in blood than does blood culture testing. |
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Keywords : SeptiFast test, Catheter-related infections, Blood culture |
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