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Original Articles
Clinical Efficacy Evaluation of Multi-parameter Realtime Polymerase Chain Reaction for the Central Venous Catheter-related Blood Stream Infection
Bongyoung Kim1*, Sewoo Park1*, Taehyung Kim1, Jieun Kim1, Donghwi Rim1, Taeyeal Choi2, Hyunjoo Pai1, and Jungoak Kang2
Departments of 1Internal Medicine and 2Laboratory Medicine, Hanyang University College of Medicine, Seoul, Korea
Vol.43 Num.3 (p240~244)
Background: The study evaluated the clinical efficacy of a multi-parameter realtime polymerase chain reaction (PCR) test for patients with central venous catheterrelated bloodstream infection (CRBSI).

Materials and Methods: Thirty five patients suspected to have CRBSI were enrolled. The SeptiFast (SF) multi-parameter real-time PCR test (Roche Diagnostics, Germany) and blood culture were performed and results were compared.

Results: The turn-around time for the SF test and blood culture was 32.628.9 hours and 115.823.5 hours, respectively. Among the 70 blood samples, the positive rates of SF test and blood culture were 34.3% and 27.1%, respectively, and the agreement rate was 62.9%. Gram-positive bacteria were detected in 10 patients with blood culture and 11 patients with SF test. Gram-negative bacteria were detected in one patient with a blood culture and in seven patients with SF test. Candida was not detected in blood culture but was detected in two patients by the SF test.

Conclusions: SF test was faster and more sensitive for the detection of blood pathogens than blood culture. It provides a more sensitive detection of gramnegative and Candida in blood than does blood culture testing.
Keywords : SeptiFast test, Catheter-related infections, Blood culture