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 Original Articles |
| Application of Infrequent-Restriction-Site Polymerase Reaction (IRS-PCR) to the Molecular Epidemiologic Analysis of Methicillin Resistant Staphylococcus aureus (MRSA) |
| Na-Young Shin1, Jin-Hong Yoo2, Chulmin Park3, Dong-Gun Lee2, Su-Mi Choi2, Jae-Cheol Kwon2, Si-Hyun Kim2, Sun-Hee Park2, and Jung-Hyun Choi2 |
| 1Department of Biomedical Science, The Catholic University of Korea, Graduate School, 2Department of Internal Medicine, College of Medicine, and 3Catholic Research Institutes of Medical Science, The Catholic University of Korea, Seoul, Korea |
Vol.43 Num.5 (p396~405)
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Background: We investigated the usefulness of infrequent-restriction-site polymerase chain reaction (IRS-PCR) compared with pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) on the molecular epidemiologic analysis of methicillin-resistant Staphylococcus aureus (MRSA).
Materials and Methods: We used fifty clinical isolates of MRSA collected from 10 university hospitals located in Seoul. We performed three procedures on these isolates: PFGE using SmaI, IRS-PCR using XbaI-Hha I or EagI-Hha I, and MLST using seven house-keeping genes. We determined the clusters of molecular types by dendrogram using the unweighted pair group method with arithmetic mean (UPGMA) and Dice coefficients
Results: MLST analysis showed that isolates exhibited ST1, ST5, ST72, ST89, and ST239. In PFGE, the isolates clustered into 5 major groups with 80% similarity, which subsequently became classified into 18 subgroups with 95% similarity. In IRS-PCR using EagI-HhaI restriction enzymes, there was little resolution among the patterns of isolates. However, Xba I-Hha I IRS-PCR showed 5 groups with a 90% similarity. These groups were then classified into 9 subgroups with a 95% similarity. There were no significant differences among the isolates from different hospitals.
Conclusions: The XbaI-HhaI IRS-PCR method could be a useful tool in the molecular epidemiology of MRSA. Its resolution power was good enough to analyze isolates, because the patterns of IRS-PCR were closely correlated with those of MLST and showed diverse groups. |
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Keywords : Methicillin resistant Staphylococcus aureus, Pulsed Field Gel Electrophoresis, Infrequent restriction site PCR, Multilocus sequence typing |
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