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 Original Articles |
| Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE) |
| Ji-Hyun Byun1, Jin-Hong Yoo2*, Chulmin Park3, Dong-Gun Lee2, Sun-Hee Park2, Su-Mi Choi2, Jung-Hyun Choi2, Si-Hyun Kim2, and Jae-Cheol Kwon2 |
| Department of Biomedical Science, The Catholic University of Korea, Graduate School1, Department of Internal Medicine2, College of Medicine, and Catholic Research Institutes of Medical Science3, The Catholic University of Korea, Seoul, Korea |
Vol.44 Num.1 (p5~10)
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Background: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli , and also assessed the use of PFGE as an alternative method.
Materials and Methods: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE.
Results: In E. coli , PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%) of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days).
Conclusions: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli. |
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Keywords : ESBL-producing E. coli , Pulsed-field gel electrophoresis (PFGE), Infrequent restriction site-polymerase chain reaction (IRS-PCR), Genotypin |
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