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 Home > Available Issues |
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 Original Articles |
| Antimicrobial Actinity against MRSA and Safety of Grapefruit Seed Extract |
| Young Ho Kim and InWha Seong |
| Department of Microbiology, College of Medicine, Korea University |
Vol.20 Num.4 (p323~331)
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Background : Inappropriate use of antibiotics has resulted in emergence of antibiotic-resistant microorganisms, increased hospital infections and urgency of developing new antibiotics coping the antibiotic-resistant microorganisms. Grapefruit seed extract has been studied for the possibility of use as antimicrobial additive to kill microorganisms in foods. In this study, grapefruit seed extract was examined for antimicrobial activity against MRSA and safety.
Methods : Serially diluted Grapefruit seed extract(DH-100) was added to the paper disks and antimicrobial activity was determined with disk diffusion method. For the safety test, 0.1㎖ , 0.2㎖, 0.3㎖ of diluted extract(5mg/㎖) were inoculated into 10 ICR mice by oral and intraperitoneal route respectively.
Result : Growth of MRSA and S. aureus was inhibited at the concentration of 0.5mg/㎖, B. subtilis at 1mg/㎖, but the extract of concentration at 1mg/㎖ did not showed antimicrobial activity against all the gram-negative bacteria, E. coli, S. typhi, P. vulgaris and P. aeruginosa. Lethal dose of grapefruit seed extract(DH-100) was above 15g/kg, Further study could show that grapefruit seed extract would be used as a safe antimicrobial agent in man.
Conclusion : Grapefruit seed extract showed antimicrobial activity against MRSAs and revealed safety in ICR mice.
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Keywords : grapeseed extract, antimicrobial activity, safety |
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